Rabu, 04 September 2019

THE SIMPLE IDENTIFICATION OF FORMALIN, BORAX AND RHODAMIN SPOT TEST METHODS USING SOME KITS TESTER PRODUCT


THE SIMPLE IDENTIFICATION  OF FORMALIN, BORAX AND RHODAMIN
SPOT TEST METHODS USING SOME KITS TESTER PRODUCT

By Chanif Mahdi*)
Discoverer and producer
Department of Chemistry faculty of science the University of Brawijaya
Malang, East of Jawa Province, Indonesia

1. FORMALIN MAIN REAGENT ( FMR)

It is one of instrument kit tester reagent to determine formalin content of food and his ingredient as a preservative or formalin contaminant. This instrument as a reagent kit tester form, in 15 ml plastic bottle packed. It can be used for formalin detection on food and food ingredients. With the procedure operation as follow.
Please one a gram of food sample in test tube, add 2-3 ml of FMR reagent, shake well for 3-5 minutes. If sample food and food ingredient to indicate positive formalin detection , the reagent solution will turn form yellow colour to pink or blue colour. The deeper of colour depend on formalin concentration on food samples. Formalin kit tester (FMR ) can ditection formalin on food with the content less than 5 ppm, in five or ten minutes periode . To know the level of concentration result of formalin  ditection, should be compare to formalin colour standart as follow.


     
                                          Figure: FMR plastic bottle 15 ml packed

                                                Figure : Colour standart  of formalin

2. BORAX MAIN REAGENT ( BMR)
                                                      
It is one of instrument reagent kit tester for borax determine or ditection  on food and food ingredient. This instrument as a reagent kit tester form, in 15 ml plastic bottle packed. This kit tester can be used for determine or ditection  borax as food additive or food preservative, with procedure operational as follow.
Please one gram of powder or grain food samples in dropplate porcelain or white plate porcelain, please add few drop of BMR reagent. If food samples  indicate  positive ditection, the colour will turn from yellow colour to cherry red colours or dark brown colour, depend on level of borax concentration on food samples. To know as  quantitative concentration of borax , we can used  standart borax colouras follow.



Figure : BMR plastic bottle 15 ml packed



Figure :  borax colour standart




3. Colours Main Reagent (CMR)


It is one of the instrument reagen kit tester for determine or ditection colour additive on food and drinks. This instrument as a reagent kit form, in 15 ml plastic bottle packed. This reagen can be used to determine and to ditect  colour additive on food and soft driks. The CMR reagent kit tester are supplemented by two reagent supplements, both are ammonia reagent and petroleum reagent. The procedure operation as follow:
  1. Please one ml or one gram sample in test tube
  2. Add 5 drops of ammonia supplement reagent
  3. Add 2 or 3 mll of petroleum eter  ( car/ motor petroleum)  as a supplement reagen
  4. Shake well between 3- 5 minute
  5. Let the solution be separated, and pour off petroleum layer into another test tube
  6. Add the petroleum phase with  2-3 ml  CMR reagen, and shake well for 3- 5 minutes
  7. Let the solution be separated 
  8. please observer to bottom layer, colour appear or not. The positive ditection if colour appear on bottom layer.


Figure : Negatif rhodamin colour ditection


Figure : Positive rhodamin colour ditection


4. YODIUM MAIN REAGENT ( YMR reagent )

It is one of instrument reagent kit tester for determine or ditection yodium content of yodium salt. This instrument as a reagent kit tester form, in 15 ml plastic bottle packed.  This reagent kit can be used to determine or ditection yodium content on yodat salt, with the procedure operation  as follow :
  1. Please one gram of powder or grain samples of salt
  2. Add few drops ( 2-3 drops) of YMR reagent
  3. If salt sample indicate positif ditection, the salt sample will appear blue colour directly.
  4. To know the quantitative borax content on salt, we can measure by using standart colour of yodium concentration.



More information please contact to Chanif Mahdi, with addres as follow:
Office : Departemen Chemistry Faculty of Science University of Brawijaya
             Malang. Indonesia  Telp/Fax : 0341 554403
Private Laboratory :  BioChem Healthy Food 
            Jalan Venus 21 Malang. Telp ( 0341 ) 553791 HP : 08125297102


REFERENCES

Anonymous, 1987. Handbook of test kit for formarlin in food. Departement of  medical
             sciences. The ministry of public health. Thailand.

Encyclopedia, 2005. Borax. The Columbia electronic encyclopedia. 6th edition.Copy
           right. Columbia University Press. All right reversed.

Erwin, G. 1985. Spot Test Analysis  Clinical Enveroment, 2nd  Edition. Book News, Inc.
           Portland..

Groenevelt, J.P., 1984. Simple test manual. Testing Departement Consumentenbond.
           Publisher  by Consumentenbond. In behalf of the International Organisation of
          Consumers Union ( ID